Genomic Signature Pattern Analysis of the HIV-1 Through Computational Approach Sushanta Kumar Barik Published: 2021-08-08 DOI: 10.17504/protocols.io.bw7sphne HIV-1 Genomic Signature Protease Reverse transcriptase AI 解读 浏览 1133 Abstract ). Steps1. 2. 3. 4. 5. 5 6. 6 7. 7 推荐阅读 Nature Protocols Protocols IO Current Protocols Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells The cell-line-derived subcutaneous tumor model in preclinical cancer research Subcellular patch-clamp techniques for single-bouton stimulation and simultaneous pre- and postsynaptic recording at cortical synapses NovoSpaRc: flexible spatial reconstruction of single-cell gene expression with optimal transport Genome-wide pooled CRISPR screening in neurospheres High-throughput cultivation and identification of bacteria from the plant root microbiota Viral crosslinking and solid-phase purification enables discovery of ribonucleoprotein complexes on incoming RNA virus genomes Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility Addendum: Micro-CT acquisition and image processing to track and characterize pulmonary nodules in mice Multiplexed single-cell proteomics using SCoPE2 查看全部 Human Neuronal Nucleus Isolation for Single-Nucleus Transcriptomic Profiling (10x Genomics) single-nucleus RNA-seq neuronal nucleus suspension human tissue spinal cord UAB-VU BIOMIC Preparation of Left Fresh Frozen Eye HuBMAP BIOMIC MSRC Vanderbilt UAB Eye Imaging eDNA extraction from buffer EtOH 100% using DNeasy Blood and Tissue Kit (QIAGEN) MERS-CoV Mpro fluorescence dose response The role of sphingolipids in the pathogenesis of psoriasis Psoriasis ceramide sphingolipid S1P-lyase Sphingosine Lipid signaling S1P-lyase Sphingosine 1-phosphate Sphingosine kinase Sphingomyelin Ceramide S1P receptor Detection of SARS CoV-2 in different samples with a singleplex and mutation resistant real time PCR assay SARS CoV-2 real time PCR Coronavirus pandemic outbreak Wuhan strain Omicron mutation TaME-seq2 integration library preparation illumina virus viral integrations snp snps variants whole genome sequencing genome genomics enrichment multiplex PCR Singleplex Assay for Fitness Measurements Deep mutational scanning protein sequence-function relationships fitness landscape laboratory automation SRA and Genbank BioSample-Linked Submission with Mercury_Prep and Mercury_Batch ACE-inhibitory activity assay: IC50 ACE angiotensin-converting enzyme antihypertensive IC50 bioactivity ngiotensin-I converting enzyme 查看全部 RNA Extraction from Plant Tissue with Homemade Acid Guanidinium Thiocyanate Phenol Chloroform (AGPC) AGPC home-made buffers molecular biology protocol multiple plant species and tissues nucleic acids RNA isolation Genotyping Microbial Communities with MIDAS2: From Metagenomic Reads to Allele Tables copy number variant genotyping microbiome single nucleotide variant strain MIDAS2 Using ExpressAnalyst for Comprehensive Gene Expression Analysis in Model and Non-Model Organisms differential expression analysis meta-analysis proteomics transcriptomics visual analytics Supervised Learning of Gene Regulatory Networks gene expression profiles gene regulatory networks supervised learning support vector machine One-Flask Synthesis of Cyclic Diguanosine Monophosphate (c-di-GMP) bacterial signaling c-di-GMP chemical synthesis Antibody-Based CAR T Cells Produced by Lentiviral Transduction CAR T cells chimeric antigen receptor lentiviral transduction Two-Tailed RT-qPCR for the Quantification of A-to-I-Edited microRNA Isoforms A-to-I editing biomarker discovery microRNA non-coding RNAs RNA editing two-tailed RT-qPCR Specific Localization and Quantification of the Oligo-Mouse-Microbiota (OMM12) by Fluorescence In Situ Hybridization (FISH) altered Schaedler flora intestine microbial ecology oligo-MM sDMDMm2 single-cell analysis spatial structure Optical Genome Mapping for Applications in Repeat Expansion Disorders optical genome mapping repeat expansion disorders somatic instability structural variant analysis whole genome analysis Single-Molecule Localization Microscopy Using Time-Lapse Imaging of Single-Antibody Labeling antibodies multiplexed imaging single-antibody labeling single-molecule localization microscopy superresolution imaging time-lapse imaging 查看全部
