GEARBOCS protocols Justin T Savage, Luke Bradley, Nicholas Brose Published: 2024-08-19 DOI: 10.17504/protocols.io.36wgqnq15gk5/v2 ASAPCRN AI 解读 浏览 1150 Abstract Protocols for AAV production and use of the GEARBOCS system. Steps 推荐阅读 Nature Protocols Protocols IO Current Protocols Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells The cell-line-derived subcutaneous tumor model in preclinical cancer research Subcellular patch-clamp techniques for single-bouton stimulation and simultaneous pre- and postsynaptic recording at cortical synapses NovoSpaRc: flexible spatial reconstruction of single-cell gene expression with optimal transport Genome-wide pooled CRISPR screening in neurospheres High-throughput cultivation and identification of bacteria from the plant root microbiota Viral crosslinking and solid-phase purification enables discovery of ribonucleoprotein complexes on incoming RNA virus genomes Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility Addendum: Micro-CT acquisition and image processing to track and characterize pulmonary nodules in mice Multiplexed single-cell proteomics using SCoPE2 查看全部 SARS-CoV-2 nsp3 macrodomain expression and purification protocol for crystallization expression purification ASAP CMD AViDD SARS-CoV-2 SARS-CoV-2 nsp3 SARS-CoV-2 nsp3 macrodomain SARS-CoV-2 macrodomain nsp3 macrodomain #nsp3 macrodomain mac1 his-tag Protocol for 3D bioprinting functional human brain tissues ASAPCRN A Supplemental Guide to Building the optoPlate-96 optoPlate-96 optoPlate optogenetics LEDs reflow soldering Optimized Protocol for RNA Extraction from Insect Samples Using TRIzol Reagent Smart-seq3xpress PCR and gel electropheresis Making Agarose for use in acute in vivo Electrophysiology Experiments Agarose Electrophysiology Neuropixels UPitt TriState SenNet TMC H&E staining histology staining Protocol for detection of Salmonella Typhi and Salmonella Paratyphi A in Surface water (and other types of water samples) Surface water water Salmonella Typhi Salmonella Paratyphi A enrichment detection environment Immunofluorescence Staining ASAPCRN 查看全部 RNA Extraction from Plant Tissue with Homemade Acid Guanidinium Thiocyanate Phenol Chloroform (AGPC) AGPC home-made buffers molecular biology protocol multiple plant species and tissues nucleic acids RNA isolation Genotyping Microbial Communities with MIDAS2: From Metagenomic Reads to Allele Tables copy number variant genotyping microbiome single nucleotide variant strain MIDAS2 Using ExpressAnalyst for Comprehensive Gene Expression Analysis in Model and Non-Model Organisms differential expression analysis meta-analysis proteomics transcriptomics visual analytics Supervised Learning of Gene Regulatory Networks gene expression profiles gene regulatory networks supervised learning support vector machine One-Flask Synthesis of Cyclic Diguanosine Monophosphate (c-di-GMP) bacterial signaling c-di-GMP chemical synthesis Antibody-Based CAR T Cells Produced by Lentiviral Transduction CAR T cells chimeric antigen receptor lentiviral transduction Two-Tailed RT-qPCR for the Quantification of A-to-I-Edited microRNA Isoforms A-to-I editing biomarker discovery microRNA non-coding RNAs RNA editing two-tailed RT-qPCR Specific Localization and Quantification of the Oligo-Mouse-Microbiota (OMM12) by Fluorescence In Situ Hybridization (FISH) altered Schaedler flora intestine microbial ecology oligo-MM sDMDMm2 single-cell analysis spatial structure Optical Genome Mapping for Applications in Repeat Expansion Disorders optical genome mapping repeat expansion disorders somatic instability structural variant analysis whole genome analysis Single-Molecule Localization Microscopy Using Time-Lapse Imaging of Single-Antibody Labeling antibodies multiplexed imaging single-antibody labeling single-molecule localization microscopy superresolution imaging time-lapse imaging 查看全部
