Doubling the resolution of a confocal spinning-disk microscope using image scanning microscopy

Shun Qin, Sebastian Isbaner, Ingo Gregor, Jörg Enderlein

Published: 2020-11-26 DOI: 10.1038/s41596-020-00408-x

Fluorescence Microscopy

Image Scanning Microscopy

Confocal Spinning-Disk Microscope

Structured Illumination

Resolution Doubling

AI 解读

Supplementary information

Supplementary Information

Supplementary Note 1, Supplementary Figs. 1–7 and Supplementary Table 1.

Supplementary Video 1

Screen-captured video explaining the use of the Micro-Manager plugin.

Supplementary Video 2

Screen-captured video explaining the use of the reconstruction software.

Supplementary Video 3

This video illustrates the data acquisition and the ISM reconstruction of the bead image in Fig. 4a,b.

Supplementary Video 4

This video illustrates the data acquisition and the ISM reconstruction of the cell image in Fig. 4g,h.

Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells

The cell-line-derived subcutaneous tumor model in preclinical cancer research

Subcellular patch-clamp techniques for single-bouton stimulation and simultaneous pre- and postsynaptic recording at cortical synapses

NovoSpaRc: flexible spatial reconstruction of single-cell gene expression with optimal transport

Genome-wide pooled CRISPR screening in neurospheres

High-throughput cultivation and identification of bacteria from the plant root microbiota

Viral crosslinking and solid-phase purification enables discovery of ribonucleoprotein complexes on incoming RNA virus genomes

Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility

Addendum: Micro-CT acquisition and image processing to track and characterize pulmonary nodules in mice

Multiplexed single-cell proteomics using SCoPE2

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References

Supplementary information

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Doubling the resolution of a confocal spinning-disk microscope using image scanning microscopy

Supplementary information

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