Design, assembly, and characterization of membrane-spanning DNA nanopores

Conor Lanphere, Daniel Offenbartl-Stiegert, Adam Dorey, Genevieve Pugh, Elena Georgiou, Yongzheng Xing, Jonathan R. Burns, Stefan Howorka

Published: 2020-12-20 DOI: 10.1038/s41596-020-0331-7

DNA nanopores

membrane-spanning

nanotechnology

chemical modification

transport assays

AI 解读

Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells

The cell-line-derived subcutaneous tumor model in preclinical cancer research

Subcellular patch-clamp techniques for single-bouton stimulation and simultaneous pre- and postsynaptic recording at cortical synapses

NovoSpaRc: flexible spatial reconstruction of single-cell gene expression with optimal transport

Genome-wide pooled CRISPR screening in neurospheres

High-throughput cultivation and identification of bacteria from the plant root microbiota

Viral crosslinking and solid-phase purification enables discovery of ribonucleoprotein complexes on incoming RNA virus genomes

Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility

Addendum: Micro-CT acquisition and image processing to track and characterize pulmonary nodules in mice

Multiplexed single-cell proteomics using SCoPE2

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DNA nanopores are bio-inspired nanostructures that control molecular transport across lipid bilayer membranes. Researchers can readily engineer the structure and function of DNA nanopores to synergistically combine the strengths of DNA nanotechnology and
Introduction
Materials
Procedure
Troubleshooting
Timing
Anticipated results
- Reporting Summary
- References
References
ACKNOWLEDGMENTS
ACKNOWLEDGMENTS

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Design, assembly, and characterization of membrane-spanning DNA nanopores

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