DToL Taxon-specific Standard Operating Procedures for Marine Metazoa

These Standard Operating Procedures (SOPs) contain guidance on how to process the various taxa that fall under the ‘Marine Metazoa’ category within the scope of the Darwin Tree of Life (DToL) project. The guidance specifically refers to the tissue samples needed for DNA barcoding (which takes place at the Natural History Museum (NHM) and at the Marine Biological Association (MBA)) and outlines the dissected tissues required for whole genome sequencing, which takes place at the Wellcome Sanger Institute. Every specimen is submitted for DNA barcoding first before potentially being sent to the Wellcome Sanger Institute. These SOPs were assembled by sampling team members from the Other Metazoa Working Group for the Darwin Tree of Life project, with contributions from experts from the Natural History Museum and the Marine Biological Association.

Please see the Guidelines for more details on how to best use these SOPs, as well as for relevant contact information and acknowledgements.

The available SOPs are listed below. Please note more may be added in future:

1- Anthozoa

2- Bivalvia

3 - Bryozoa

4 - Chitons

5 - Cephalopoda

6 - Colonial Ascidiacea

7.1 - Crustacea - Decapoda

7.2 - Crustacea - Peracarida

7.3 - Crustacea - Cirripedia

8 - Echinodermata

9 - Gastropoda

10 - Medusozoa and Ctenophora

11 - Polychaeta

12 - Porifera

13 - Pycnogonida

14 - Sipuncula

15 - Unitary Ascidiacea