Cylinder Test

Ted Dawson, Xiaobo Mao, Ramhari Kumbhar, Hanseok Ko, Valina L. Dawson

Published: 2024-03-15 DOI: 10.17504/protocols.io.5qpvo3jmzv4o/v1

Abstract

The Cylinder test is used to evaluate locomotor asymmetry and spontaneous movement in rodent models.

Steps

1.

Set up small, transparent cylinder in front of video recorder.

2.

Place mouse in cylinder and record spontaneous activity for 10 min.

3.

Clean cylinder in between mice.

4.

For all recordings, view in slow motion and count the number of forepaw touches to the cylinder walls, rears, and grooming bouts.

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