Coating plates with Matrigel for ES/iPS culture
Jiuchun Zhang, Harper JW
Abstract
This protocol is about coating plates with matrigel.
Attachments
Steps
Chill 1X On ice.
Take culture dishes you want to coat out of their package and place them inside a tissue culture hood.
Thaw desired number of Matrigel aliquots either On ice or at Room temperature.
Resuspend matrigel with chilled PBS. You want to use 25mL per 2mg.
Make sure the matrigel is well mixed in the PBS. Then aliquot proper volume into each culture dish. You will need 6mL per 10 cm dish, 1mL per well of a 6 well plate, 0.5mL per well of a 12 well plate.
Swirl plate to coat all the surface.
These plates are ready to use after 1h 0m 0s at Room temperature. Depending on the storage space situation. You can keep these plates in a 37°C tissue culture incubator, somewhere clean at 37Room temperature or in the fridge. As long as they are kept sterile and the solution is not dried out, they are good for a couple months. Dried out plates have to be either discarded or re-coated.
After 1h 0m 0s, add 2mL on top to avoid the wells from drying out.
