Cell Viability Assessment
Hanseok Ko, Valina L. Dawson, Bo Am Seo, Ted M. Dawson
Published: 2024-03-14 DOI: 10.17504/protocols.io.n92ldmd9ol5b/v1
Abstract
Assess neuronal viability.
Steps
1.
Stain neurons with Hoechst 33342 7micromolar (µM) ) and propidium iodide (PI) 2micromolar (µM) ) (Invitrogen) at37°C for0h 5m 0s .
2.
Capture images by a microscope and dead cells are automatically counted by Axiovision 4.6 software (Carl Zeiss).
3.
Subject background signals of the control group intensity.
4.
Calculate the neuronal death percentage by dividing the PI signal by the Hoechst 33342 signal.
