Cell DIVE Platform | Accessing HuBMAP Datasets and Analysis on Globus

Liz McDonough, Christine Surrette

Published: 2023-04-26 DOI: 10.17504/protocols.io.14egn2qwpg5d/v1

Abstract

This method describes navigating to Globus from the HuBMAP data portal to access Cell DIVE data and analysis.

Steps

1.

Click on the DOI link provided, or alternatively navigate to http://dx.doi.org, enter the DOI in the text box, and click Go .

2.

If the DOI takes you to a Collection Collection on the HuBMAP Data Portal, click on a HuBMAP ID of interest, located under the Dataset heading.

Image 1. Example Dataset listing from a Cell DIVE HuBMAP Collection.
Image 1. Example Dataset listing from a Cell DIVE HuBMAP Collection.
3.

On the Dataset page, scroll down to the Files heading and click the provided link to Globus.

Image 2. A link to the dataset on Globus is provided under the Files heading of the Dataset page on the HuBMAP portal.
Image 2. A link to the dataset on Globus is provided under the Files heading of the Dataset page on the HuBMAP portal.
4.

Log in to Globus using your existing organizational login e.g., university, national lab, facility, project, or eRA Commons ID.

Image 3. Overview of the Globus log in page.
Image 3. Overview of the Globus log in page.
5.

The Cell DIVE datasets are organized under two top level directories, data_directory and extras . The data_directory contains OME-TIFF data organized by region. The extras folder contains additional files including virtual H&Es and analysis data.

Image 4. High level overview of a Cell DIVE dataset on Globus.
Image 4. High level overview of a Cell DIVE dataset on Globus.
6.

To access analysis, click through to the extras/Seg_Reg sublevel directory. Analysis is organized by biomarker.

Image 5. Example contents of a Seg_Reg folder within the extras directory of a Cell DIVE dataset on Globus.
Image 5. Example contents of a Seg_Reg folder within the extras directory of a Cell DIVE dataset on Globus.

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