Cas9 Protein Purification Protocol
Shu Huang
Published: 2024-08-06 DOI: 10.17504/protocols.io.3byl49rpjgo5/v1
Abstract
This protocol outlines the steps for purifying Cas9 protein, essential for the preparation of RNP complexes used in CRISPR-Cas9 editing processes.
Steps
Expression of Cas9 protein in a suitable host system
1.
Express the Cas9 protein in a host system that supports optimal protein yield and stability.
Lysis and purification of Cas9 protein
2.
Lyse the host cells and purify the Cas9 protein using standard biochemical techniques to ensure high purity and activity.
Quality assessment of purified protein
3.
Assess the quality of the purified Cas9 protein using appropriate assays to verify its activity and suitability for further applications.
