CPMU
Bjorn Bartholdy, a.g.henry
Published: 2022-12-09 DOI: 10.17504/protocols.io.n92ld96dng5b/v2
Abstract
Mineralising solution for the oral biofilm. From Sissons et al. 1991.
Before start
This solution MUST be prepared in a sterile and starch-free environment.
Steps
1.
Add 300mL distilled (or deionized) dH2O to a 1000mL beaker, with stirring and heat 60°C
2.
Add:
-
1.55g -
1.44g -
0.72g -
0.08g -
30g
3. The solution should be filter-sterilised before storing!
Add the remaining 700mL and keep stirring until precipitate has completely dissolved
Store in fridge at 4°C
Note
