Assembly of complete mouse embryo models from embryonic and induced stem cell types in vitro Kasey Y. C. Lau, Gianluca Amadei, Magdalena Zernicka-Goetz Published: 2023-10-10 DOI: 10.1038/s41596-023-00891-y Embryo models Induced stem cells Gastrulation Mouse embryogenesis Stem cell assembly AI 解读 浏览 1115 PDFMetadata 暂无数据 推荐阅读 Nature Protocols Protocols IO Current Protocols Genetic manipulation and immortalized culture of ex vivo primary human germinal center B cells The cell-line-derived subcutaneous tumor model in preclinical cancer research Subcellular patch-clamp techniques for single-bouton stimulation and simultaneous pre- and postsynaptic recording at cortical synapses NovoSpaRc: flexible spatial reconstruction of single-cell gene expression with optimal transport Genome-wide pooled CRISPR screening in neurospheres High-throughput cultivation and identification of bacteria from the plant root microbiota Viral crosslinking and solid-phase purification enables discovery of ribonucleoprotein complexes on incoming RNA virus genomes Mitochondrial single-cell ATAC-seq for high-throughput multi-omic detection of mitochondrial genotypes and chromatin accessibility Addendum: Micro-CT acquisition and image processing to track and characterize pulmonary nodules in mice Multiplexed single-cell proteomics using SCoPE2 查看全部 Human Neuronal Nucleus Isolation for Single-Nucleus Transcriptomic Profiling (10x Genomics) single-nucleus RNA-seq neuronal nucleus suspension human tissue spinal cord UAB-VU BIOMIC Preparation of Left Fresh Frozen Eye HuBMAP BIOMIC MSRC Vanderbilt UAB Eye Imaging eDNA extraction from buffer EtOH 100% using DNeasy Blood and Tissue Kit (QIAGEN) MERS-CoV Mpro fluorescence dose response The role of sphingolipids in the pathogenesis of psoriasis Psoriasis ceramide sphingolipid S1P-lyase Sphingosine Lipid signaling S1P-lyase Sphingosine 1-phosphate Sphingosine kinase Sphingomyelin Ceramide S1P receptor Detection of SARS CoV-2 in different samples with a singleplex and mutation resistant real time PCR assay SARS CoV-2 real time PCR Coronavirus pandemic outbreak Wuhan strain Omicron mutation TaME-seq2 integration library preparation illumina virus viral integrations snp snps variants whole genome sequencing genome genomics enrichment multiplex PCR Singleplex Assay for Fitness Measurements Deep mutational scanning protein sequence-function relationships fitness landscape laboratory automation SRA and Genbank BioSample-Linked Submission with Mercury_Prep and Mercury_Batch ACE-inhibitory activity assay: IC50 ACE angiotensin-converting enzyme antihypertensive IC50 bioactivity ngiotensin-I converting enzyme 查看全部 RNA Extraction from Plant Tissue with Homemade Acid Guanidinium Thiocyanate Phenol Chloroform (AGPC) AGPC home-made buffers molecular biology protocol multiple plant species and tissues nucleic acids RNA isolation Genotyping Microbial Communities with MIDAS2: From Metagenomic Reads to Allele Tables copy number variant genotyping microbiome single nucleotide variant strain MIDAS2 Using ExpressAnalyst for Comprehensive Gene Expression Analysis in Model and Non-Model Organisms differential expression analysis meta-analysis proteomics transcriptomics visual analytics Supervised Learning of Gene Regulatory Networks gene expression profiles gene regulatory networks supervised learning support vector machine One-Flask Synthesis of Cyclic Diguanosine Monophosphate (c-di-GMP) bacterial signaling c-di-GMP chemical synthesis Antibody-Based CAR T Cells Produced by Lentiviral Transduction CAR T cells chimeric antigen receptor lentiviral transduction Two-Tailed RT-qPCR for the Quantification of A-to-I-Edited microRNA Isoforms A-to-I editing biomarker discovery microRNA non-coding RNAs RNA editing two-tailed RT-qPCR Specific Localization and Quantification of the Oligo-Mouse-Microbiota (OMM12) by Fluorescence In Situ Hybridization (FISH) altered Schaedler flora intestine microbial ecology oligo-MM sDMDMm2 single-cell analysis spatial structure Optical Genome Mapping for Applications in Repeat Expansion Disorders optical genome mapping repeat expansion disorders somatic instability structural variant analysis whole genome analysis Single-Molecule Localization Microscopy Using Time-Lapse Imaging of Single-Antibody Labeling antibodies multiplexed imaging single-antibody labeling single-molecule localization microscopy superresolution imaging time-lapse imaging 查看全部
