10x v3.1 HT Genomics Sample Processing Protocol

Allen Institute

Published: 2024-05-21 DOI: 10.17504/protocols.io.ewov19o6klr2/v1

Abstract

Protocol is used for rapid generation of 3’ transcriptomic-NGS-ready- single-cell-libraries from pools of cells. Doubling the capacity of the original 10xv3.1 protocol, allowing for a capture of ~20,0000 cells instead of ~10,000 cells per port.

Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number

1U01MH114812-01

1U19MH114830-01. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.

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