质粒库
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名称 | 描述 | 操作 |
---|---|---|
pGuide | - | 查看 |
pGuide-it-sgRNA1 Vector (Linear) | Linearized CRISPR/Cas9 vector with an improved scaffold, for cloning and expression of a single guide RNA (sgRNA). | 查看 |
pGuide-it-tdTomato Vector (Linear) | Linearized CRISPR/Cas9 vector with a tdTomato marker, for cloning and expression of a single guide RNA (sgRNA). | 查看 |
pGuide-it-ZsGreen1 Vector (Linear) | Linearized CRISPR/Cas9 vector with a ZsGreen1 marker, for cloning and expression of a single guide RNA (sgRNA). | 查看 |
pH3c-LIC | Bacterial vector for expressing a protein with an N-terminal 8xHis tag followed by an HRV 3C protease cleavage site. | 查看 |
pH3c-LIC (linearized) | Linearized bacterial vector for ligation-independent cloning (LIC), with an N-terminal 8xHis tag followed by an HRV 3C protease cleavage site. | 查看 |
pH6HTC His6HaloTag T7 | Vector with a traditional MCS, for bacterial or cell-free expression of a protein with a cleavable C-terminal HaloTag®-6xHis dual tag. | 查看 |
pH6HTN His6HaloTag T7 | Vector with a traditional MCS, for bacterial or cell-free expression of a protein with a cleavable N-terminal 6xHis-HaloTag® dual tag. | 查看 |
pHAGE EF1-alpha dCas9-KRAB | Lentiviral CRISPR interference vector for constitutive expression of catalytically inactive dCas9 fused to a KRAB transcriptional repressor domain. | 查看 |
pHAGE EF1-alpha dCas9-VP64 | Lentiviral CRISPR activation vector for constitutive expression of catalytically inactive dCas9 fused to the VP64 transcriptional activation domain. | 查看 |
pHAGE-TO-dCas9-3XGFP | Lentiviral vector for labeling chromosomal loci by tetracycline-regulatable expression of catalytically inactive dCas9 fused to three copies of GFP. | 查看 |
pHAGE-TO-dCas9-3XmCherry | Lentiviral vector for labeling chromosomal loci by tetracycline-regulatable expression of catalytically inactive dCas9 fused to three copies of mCherry. | 查看 |
pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2 | Lentiviral vector for labeling chromosomal loci by tetracycline-regulatable expression of catalytically dead Streptococcus thermophilus dCas9 fused to three copies of mTagBFP2. | 查看 |
pHAGE-TO-nmdCas9-3XGFP | Lentiviral vector for labeling chromosomal loci by tetracycline-regulatable expression of catalytically dead Neisseria meningitidis dCas9 fused to three copies of GFP. | 查看 |
pHAGE-TO-nmdCas9-3XmCherry | Lentiviral vector for labeling chromosomal loci by tetracycline-regulatable expression of catalytically dead Neisseria meningitidis dCas9 fused to three copies of mCherry. | 查看 |
pHAGE TRE dCas9 | Lentiviral CRISPR activation vector for doxycycline-inducible expression of catalytically inactive dCas9. | 查看 |
pHAGE TRE dCas9-KRAB | Lentiviral CRISPR interference vector for doxycycline-inducible expression of catalytically inactive dCas9 fused to a KRAB transcriptional repressor domain. | 查看 |
pHAGE TRE dCas9-VP64 | Lentiviral CRISPR activation vector for doxycycline-inducible expression of catalytically inactive dCas9 fused to the VP64 transcriptional activation domain. | 查看 |
pHANNIBAL | Vector for silencing genes in plants using intron-containing hairpin RNA (hpRNA). | 查看 |
phCMV1 | Compact mammalian cell vector for high-level expression from an optimized CMV promoter. | 查看 |