质粒库
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名称 | 描述 | 操作 |
---|---|---|
pCS2+hSpCas9 | Plasmid for in vitro transcription of human codon-optimized S. pyogenes Cas9 using the SP6 promoter. | 查看 |
pCS2-nCas9n | Plasmid for expressing zebrafish codon-optimized S. pyogenes Cas9 with N- and C-terminal nuclear localization signals. | 查看 |
pCS6-Empty | Empty cloning vector for transient mammalian cell expression of a cDNA. Related to pCMV SPORT6. | 查看 |
pCTAP-A | Vector for expressing proteins C-terminally tagged with streptavidin- and calmodulin-binding peptides. For other reading frames, use pCTAP-B or pCTAP-C. | 查看 |
pCTAP-B | Vector for expressing proteins C-terminally tagged with streptavidin- and calmodulin-binding peptides. For other reading frames, use pCTAP-A or pCTAP-C. | 查看 |
pCTAP-C | Vector for expressing proteins C-terminally tagged with streptavidin- and calmodulin-binding peptides. For other reading frames, use pCTAP-A or pCTAP-B. | 查看 |
pCW-Cas9 | Lentiviral vector for doxycycline-inducible expression of FLAG®-tagged Cas9. | 查看 |
pd1EGFP-N1 | Vector for fusing destabilized EGFP, with an in vivo half-life of ~1 hour, to the C-terminus of a partner protein. | 查看 |
pd2ECFP-N1 | Vector for fusing destabilized ECFP, with an in vivo half-life of ~2 hours, to the C-terminus of a partner protein. | 查看 |
pd2EGFP-N1 | Vector for fusing destabilized EGFP, with an in vivo half-life of ~2 hours, to the C-terminus of a partner protein. | 查看 |
pd2EYFP-N1 | Vector for fusing destabilized EYFP, with an in vivo half-life of ~2 hours, to the C-terminus of a partner protein. | 查看 |
pd4EGFP-N1 | Vector for fusing destabilized EGFP, with an in vivo half-life of ~4 hours, to the C-terminus of a partner protein. | 查看 |
pDB.GST | Bacterial expression vector with a kanamycin resistance marker, for appending an N-terminal GST tag followed by a TEV site. | 查看 |
pDB-HisGST | Bacterial expression vector with a kanamycin resistance marker, for appending an N-terminal 6xHis-GST tag followed by a TEV site. | 查看 |
pDB.His.MBP | Bacterial expression vector with a kanamycin resistance marker, for appending an N-terminal 6xHis-MBP tag followed by a TEV site. | 查看 |
pDB.His.NusA | Bacterial expression vector with a kanamycin resistance marker, for appending an N-terminal 6xHis-NusA tag followed by a TEV site. | 查看 |
pDB.His.TRX | Bacterial expression vector with a kanamycin resistance marker, for appending an N-terminal 6xHis-thioredoxin tag followed by a TEV site. | 查看 |
pDB.TRX | Bacterial expression vector with a kanamycin resistance marker, for appending an N-terminal thioredoxin tag followed by a TEV site. | 查看 |
pdCas9 | Bacterial plasmid for expressing catalytically inactive dCas9 together with a crRNA precursor, for transcriptional repression. | 查看 |
pdCas9-bacteria | Bacterial plasmid for tetracycline-inducible expression of catalytically dead S. pyogenes Cas9. | 查看 |