质粒库
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名称 | 描述 | 操作 |
---|---|---|
pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B | - | 查看 |
pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6 | - | 查看 |
pU6-BbsI-chiRNA | Plasmid for expressing a chimeric RNA in Drosophila cells from the U6 promoter. Use together with pHsp70-Cas9. | 查看 |
PU6_klp-12_sgRNA | C. elegans plasmid for expressing a single guide RNA (sgRNA). Use together with Peft-3::cas9-SV40_NLS::tbb-2 3''UTR. | 查看 |
pUAST | P element-based vector for Gal4-regulated expression of genes in Drosophila. | 查看 |
pUC118 | Cloning vector with a phage origin for producing single-stranded DNA. The MCS is reversed in pUC119. | 查看 |
pUC119 | Cloning vector with a phage origin for producing single-stranded DNA. The MCS is reversed in pUC118. | 查看 |
pUC119-gRNA | CRISPR plasmid for expressing a single guide RNA (sgRNA) in plant cells. Use together with HBT-pcoCas9. | 查看 |
pUC18 | Standard E. coli vector with a multiple cloning site (MCS) for DNA cloning. The MCS is reversed in pUC19. | 查看 |
pUC19 | Standard E. coli vector with a multiple cloning site (MCS) for DNA cloning. | 查看 |
pUC19 | Standard E. coli vector with a multiple cloning site (MCS) for DNA cloning. The MCS is reversed in pUC18. | 查看 |
pUC19L | Linearized version of pUC19 for use with the GeneArt® Seamless Cloning and Assembly Kit. | 查看 |
pUC57 | Cloning vector with an ampicillin resistance marker, suitable for generating ExoIII deletions. | 查看 |
pUC57-Kan | Cloning vector with a kanamycin resistance marker, suitable for generating ExoIII deletions. | 查看 |
pUC57-sgRNA expression vector | Plasmid for in vitro transcription of a single guide RNA (sgRNA) using the T7 promoter. | 查看 |
pUC57-Simple | Derivative of the standard cloning vector pUC57 with only an EcoRV site available for cloning. | 查看 |
pUC57-T | TA cloning vector created by linearizing pUC57 with EcoRV and adding 3''-T overhangs. | 查看 |
pUCATPH | Hygromycin resistance plasmid for restriction enzyme-mediated integration (REMI) mutagenesis of fungi. | 查看 |
pUCmu | Minimal 1669-bp cloning vector derived from pUC18, with an ampicillin resistance marker. See also pICOz. | 查看 |
Puro-Cas9 donor | Vector for targeting a tetracycline-inducible Cas9 cassette to the adeno-associated virus integration site (AAVS1) in the human PPP1R12C gene. | 查看 |