质粒库
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筛选
| 名称 | 描述 | 操作 |
|---|---|---|
| pBluescript SK(-) | Standard cloning vector (phagemid excised from lambda ZAP). The f1 (–) orientation allows rescue of antisense strand ssDNA. pBluescript KS(–) has a reversed MCS. | 查看 |
| pBluescript SK(+) | Standard cloning vector (phagemid excised from lambda ZAP). The f1 (+) orientation allows rescue of sense strand ssDNA. pBluescript KS(+) has a reversed MCS. | 查看 |
| pBR322 | First-generation E. coli vector for DNA cloning. This plasmid has a low copy number (~20 copies per cell) due to the rop gene. | 查看 |
| pBS(-) | Phagemid cloning vector, also known as BlueScribe M13 Minus or BlueM13m or pBSM13(–). | 查看 |
| pBS(+) | Phagemid cloning vector, also known as BlueScribe M13 Plus or BlueM13p or pBSM13(+). | 查看 |
| pBS KS(-) | Phagemid cloning vector, also known as BlueScribe KS Minus or BlueKSm. The MCS is reversed relative to pBS SK(–). | 查看 |
| pBS KS(+) | Phagemid cloning vector, also known as BlueScribe KS Plus or BlueKSp. The MCS is reversed relative to pBS SK(+). | 查看 |
| pBSK(+) Simple-Amp | Subcloning vector derived from pBluescript II SK(+), with most of the restriction sites removed from the MCS. See also pBSK(+) Simple-Kan. | 查看 |
| pBSK(+) Simple-Kan | Subcloning vector derived from pBluescript II SK(+), with most of the restriction sites removed from the MCS and with a kanamycin resistance marker. See also pBSK(+) Simple-Amp. | 查看 |
| pBS SK(-) | Phagemid cloning vector, also known as BlueScribe SK Minus or BlueSKm. The MCS is reversed relative to pBS KS(–). | 查看 |
| pBS SK(+) | Phagemid cloning vector, also known as BlueScribe SK Plus or BlueSKp. The MCS is reversed relative to pBS KS(+). | 查看 |
| pcDNA3 | Mammalian expression vector with the CMV promoter and a neomycin-resistance marker. | 查看 |
| pcDNA3.1(-) | Mammalian expression vector with the CMV promoter. The MCS is in the reverse (–) orientation. | 查看 |
| pcDNA3.1(+) | Mammalian expression vector with the CMV promoter. The MCS is in the forward (+) orientation. | 查看 |
| pCold I | Vector for cold shock-induced expression in E. coli of a protein with a translation enhancing element followed by a 6xHis tag and a Factor Xa site. | 查看 |
| pCold II | Vector for cold shock-induced expression in E. coli of a protein with a translation enhancing element followed by a 6xHis tag. | 查看 |
| pCold III | Vector for cold shock-induced expression in E. coli of a protein with a translation enhancing element. | 查看 |
| pCold IV | Vector for cold shock-induced expression of a protein in E. coli. | 查看 |
| pCold TF | Vector for cold shock-induced expression in E. coli of a protein fused to the Trigger Factor chaperone. | 查看 |
| pCR-XL-2-TOPO | Parental vector for TOPO® cloning of long blunt-ended PCR products. | 查看 |