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名称 | 描述 | 操作 |
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Allele TA Vector (linearized) | Linearized vector with 3''-T overhangs and multiple flanking restriction sites for TA cloning of PCR products. | 查看 |
pBAD Thio-TOPO (linearized) | Linearized vector with 3''-T overhangs and bound topoisomerase, for TOPO® TA cloning and arabinose-inducible expression of a His-Patch thioredoxin fusion protein. | 查看 |
pBAD-TOPO (linearized) | Linearized vector with 3''-T overhangs and bound topoisomerase, for TOPO® TA cloning and arabinose-inducible expression. | 查看 |
pBlue-TOPO (linearized) | Linearized vector with 3''-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products to measure promoter activity using β-galactosidase. | 查看 |
pcDNA3.1 CT-GFP-TOPO (linearized) | Linearized vector with 3''-T overhangs and bound topoisomerase, for TOPO® TA cloning to add a C-terminal GFP tag. | 查看 |
pcDNA3.1 NT-GFP-TOPO (linearized) | Linearized vector with 3''-T overhangs and bound topoisomerase, for TOPO® TA cloning to add an N-terminal GFP tag. | 查看 |
pcDNA3.1 V5-His-TOPO (linearized) | Linearized mammalian vector with 3''-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products and expression of C-terminally V5- and 6xHis-tagged proteins. | 查看 |
pcDNA3.3-TOPO (linearized) | Linearized mammalian vector with 3''-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products and expression with the CMV promoter. | 查看 |
pcDNA3.4-TOPO (linearized) | Linearized mammalian vector with 3''-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products and high level expression. | 查看 |
pcDNA4 HisMax-TOPO (linearized) | Linearized mammalian vector with 3''-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products and expression of N-terminally 6xHis-tagged proteins. | 查看 |
pcDNA5 FRT TO-TOPO (linearized) | Linearized vector with 3''-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products, FLP recombination, and tetracycline-inducible expression. | 查看 |
pcDNA5 FRT V5-His-TOPO (linearized) | Linearized mammalian vector with 3''-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products, FLP recombination, and expression of tagged proteins. | 查看 |
pcDNA6.2 C-EmGFP-GW TOPO (linearized) | Linearized vector with 3''-T overhangs and bound topoisomerase, for TOPO® TA cloning to add a C-terminal GFP tag and create a Gateway® expression clone. | 查看 |
pcDNA6.2 C-YFP-GW TOPO (linearized) | Linearized vector with 3''-T overhangs and bound topoisomerase, for TOPO® TA cloning to add a C-terminal YFP tag and create a Gateway® expression clone. | 查看 |
pcDNA6.2 N-EmGFP-GW TOPO (linearized) | Linearized vector with 3''-T overhangs and bound topoisomerase, for TOPO® TA cloning to add an N-terminal GFP tag and create a Gateway® expression clone. | 查看 |
pCR2.1-TOPO (linearized) | Linearized vector with 3''-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products. Similar to pCR®II-TOPO® but lacking an SP6 promoter. | 查看 |
pCR4-TOPO (linearized) | Linearized vector with 3''-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products. Can be used to generate nested deletions for sequencing. | 查看 |
pCR8 GW TOPO (linearized) | Linearized vector with 3''-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products to create a Gateway® entry vector. | 查看 |
pCRII-TOPO (linearized) | Linearized vector with 3''-T overhangs and bound topoisomerase, for TOPO® TA cloning of PCR products. Similar to pCR®2.1-TOPO® but with an SP6 promoter. | 查看 |
pCR-XL-TOPO (linearized) | Linearized vector with 3''-T overhangs and bound topoisomerase, for TOPO® TA cloning of long PCR products. Positive clones disrupt a lethal lacZα-ccdB fusion gene. | 查看 |