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换一换Mime-seq 2.0: a method to sequence microRNAs from specific mouse cell types
Ariane Mandlbauer, Qiong Sun, Niko Popitsch, Tanja Schwickert, Miroslava Spanova, Jingkui Wang, Stefan Ameres, Meinrad Busslinger, Luisa Cochella
The description of precise miRNA expression patterns is crucial to understand what these small RNAs contribute to animal development and physiology. High-throughput sequencing of miRNAs from individual developmental stages has provided insight into temporal regulation but often lacks the cellular resolution to link miRNA-function to the biology of distinct cell types within complex tissues. Here, we provide a protocol for microRNome by methylation-dependent sequencing for mice (mime-seq 2.0). Mime-seq takes advantage of a chemical small RNA-tagging approach followed by chemo-selective, high-throughput sequencing that enables the identification of tissue- and cell type-specific miRNA profiles in animals in a sensitive and reproducible manner.
AI 解读Ultra-deep ATAC-seq for sorted neurons
Andrea Kriz, Alisa Mo
Isolation of neurons from frozen post-mortem human brain tissue and preparation of ATAC-seq libraries for ultra-deep sequencing and somatic mutation detection.
AI 解读Electrophysiological recording from Brain Slices Protocol
HaiDun
This protocol details the steps for performing slice electrophysiological recordings with DART.
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