DescriptionThe SMALP, among all synthetic nanodisc products has been developed for for the solubilization of membrane proteins from all kinds of cell membranes and subsequent isolation & purification of said membrane proteins. After dissolving SMALP 200 + buffer powder in dH2O, the product is ready-to-use. SMALP 200 has a yellowish color in solution. After successfull solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommed to use the Rho1D4-tag. Cube Biotech offers the matching products for this purpose.DatasheetsSMALP 200 DatasheetSolubilization Protocol with Copolymers & DetergentsFEATURE StateLyophilized Powder, to be solved with waterPolymer styrene-to-maleic anhydride ratio2:1Molecular weight6,500 DapH after solving7.5Solubility40 %Divalent cationic tolerance< 5 mMColorYellowishOdorOdorlessFreeze-thaw stable?YesShipping Temperatureambient temperatureStorage of lyophilized copolymer-20°C for several yearsStorage of dissolved copolymer2-8°C for several daysOther noteworthy featureMost commonly used SMAStructure  CitationsSTABILIZED PROTEINYEARAUTHORBacterial Membrane ProteinsNoh I., Guo Z., Zhou J., Gao W., Fang R.H., Zhang L.-Hernandez L.M.R. & Levental I.Alpha-Toxin degrading proteinsSun L., Wang D., Noh R., Fang H., Gao W., Zhang L.ATP-binding cassette (ABC) transporterDimitrova V.A., Song S., Karagiaridi A., Marand A., Pinkett H.W.Env-protein of HIVZhou R., Zhang S., Nguyen H.T., Ding H., Gaffney A., Kappes J.C., Smith III. A.B.S, Sodroski J.G. Lab ResultsSMALP Screening: Different membrane protein usually perform different under different SMAs variants respectively. To ease the resulting screening process we compiled all of our SMAs into a convenient SMALP Screening Set. Furthermore if you like to compare proteins's performance between SMA and DIBMA in synthetic nanodiscs, we also offer our, that includes both our SMA and DIBMA products. Figure 1: Western Blot of a purified membrane protein after stabilization through SMA. Reproduced with permission from Ms S. Nestorow, University of Birmingham.